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My System has affect Rootkit.Sirefef.Gen Virus. So i want to know which antivirus software is used to remove Rootkit.Sirefef.Gen virus file.

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Rootkit.Sirefef.Gen is not a specific specimen of malware as such. The ‘.Gen’ typically means the AV scanner has picked it up through generic heuristic algorithms as something that looks like Sirefef (ZeroAccess) rather than 100% matching a specific signature.

There is a large extended family of trojans that would trigger a detection like this and no way to know what exact strain you have, so no way to tell for sure what, if any, AV would reliably remove it. Because this is a well-known family you would hope that most competent AV should be able to remove it, but it's always possible you have some variant that differs and might not be 100% removed - and you would have no way of knowing.

As usual, if your machine has been compromised the only certain way to recover it is to reinstall the OS from scratch.

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