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@PeterA.Schneider there are many possible ways. For example, since most anti-viruses already include the file system driver intercepting file operations, such driver may simply reset the flag in the internal database of already scanned files.
You can ask for whatever you want, including DNA test. How many people would agree to this is another question. Fake IDs are not uncommon among underage drinkers either.
Yes, one can build such an application which is installed on a computer. However if it only runs for a very short time frame, it might not collect enough traffic information to make the correct decision.